FuGENE HD Transfection Reagent from Promega

Product Specs
ItemFuGENE HD Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2312
Quantity5 x 1ml
Molecular Weight~58kDa
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organismsNat CommunDecember 19, 2023Francesca Pennacchietti, Jonatan Alvelid, Rodrigo A. Morales, Martina Damenti, Dirk Ollech, Olena S. Oliinyk, Daria M. Shcherbakova, Eduardo J. Villablanca, Vladislav V. Verkhusha, Ilaria Testa8-well μSlide (Ibidi). After 24 h, cells were transfected using FuGENE transfection reagent (Promega, E2312) according to the manufacturer’s protocol. At 24–72 h after transfection, cells were washed in phosphate-buffered

(2) Single nucleotide polymorphisms rs148582811 regulates its host gene ARVCF expression to affect nicotine-associated hippocampus-dependent memoryiScienceDecember 15, 2023Zhongli Yang, Jiali Chen, Haijun Han, Yan Wang, Xiaoqiang Shi, Bin Zhang, Ying Mao, Andria N. Li, Wenji Yuan, Jianhua Yao, et al.see Table S2",This study,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pRL-TK,Promega,E2312 pGL4.26,Promega,E8441 pcDNA3.1,Invitrogen,V80020 XRCC5-siRNA,GenePharma,N/A Plasmid: PX459,Addgene,Plasmid

(3) A versatile method for the identification of senolytic compoundsCell StressDecember 11, 2023Chiara Annunziata, Francesca Castoldi, Schlegel Jan, Hazel X. Ang, Mina Ristovska, Stefania Melini, Robert Welch, Christian G. Riedel, Federico PietrocolaID: 12260) and 2 µg pMD2.G (Addgene, ID 12259) plasmids using the FuGENE HD transfection reagent (#E2312, Promega). The supernatant was filtered through a 0.45 µm membrane filter and used to infect recipient

(4) A synthetic biology approach defines mechanistic and regulatory roles for phase condensation in the assembly of microtubule architectures by human TPPPbioRxivDecember 6, 2023Chih-Chia Chang, Scott M. Coyletransgene expression vector and the viral packaging plasmids psPAX2 and pMD2.G using Fugene HD (Promega #E2312). Viral production was performed in 6-well tissue culture treated plates (Corning 3335). Viral supernatants

(5) Variants in the CETP gene affect levels of HDL cholesterol by reducing the amount, and not the specific lipid transfer activity, of secreted CETPPLoS OneDecember 1, 2023Åsa Schawlann Ølnes, Marianne Teigen, Jon K. Laerdahl, Trond P. Leren, Thea Bismo Strøm, Katrine BjuneCell Cultures, Wiltshire, UK) were reverse transfected at 30% confluence for 24 h using FuGENE® HD (E2312; Promega, Madison, WI) according to the manufacturer’s instructions. Cell cultures HEK293 cells were

(6) Protocol for arrayed gRNA screening by base editors in mammalian cell lines using lentiviral systemSTAR ProtocolsDecember 1, 2023Nithin Sam Ravi, Anila George, Kumarasamypet M. Mohankumar09655 DMEM,HyClone,CAT # SH30243.01 Opti-MEM reduced serum media,Gibco,CAT # 31985062 FuGENE HD,Promega,CAT # E2312 LB agar,HiMedia,CAT # M1151 LB broth,HiMedia,CAT # M1245 Ampicillin sodium salt,SRL,CAT # 61314 QuickExtract

(7) Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasisiScienceNovember 17, 2023Natalia A. Szulc, Małgorzata Piechota, Lilla Biriczová, Pankaj Thapa, Wojciech PokrzywaHA-ubiquitin (18712, Addgene). Transfection was carried out using the FuGENE HD Transfection Reagent (E2312, Promega) according to the manufacturer’s guidelines, in the Opti-MEM I Reduced Serum Medium, no phenol red

(8) Proximity Labeling Expansion Microscopy (PL-ExM) resolves structure of the interactomebioRxivNovember 17, 2023Sohyeon Park, Xiaorong Wang, Xiangpeng Li, Xiao Huang, Katie C. Fong, Clinton Yu, Arthur A. Tran, Lorenzo Scipioni, Zhipeng Dai, Lan Huang, et al.transgene expression vector and viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). At 48 hours, the viral supernatant was harvested, filtered through a 0.45 μm filter (Millipore #HAWP04700),

(9) The programmed death ligand 1 interactome demonstrates bidirectional signaling coordinating immune suppression and cancer progression in head and neck squamous cell carcinomaJNCI: Journal of the National Cancer InstituteNovember 8, 2023Cera Nieto, Bettina Miller, Nathaniel Alzofon, Tugy Chimed, Jack Himes, Molishree Joshi, Karina Gomez, Farshad N Chowdhury, Phuong N Le, Alice Weaver, et al.(DMEM) (BioID2 Media). BioID2 plasmids were transfected in BioID2 Media for 24 hours (FuGene, Promega E2312), followed by 50 uM biotin (LifeTechnologies 20217) for 16 hours. Samples were prepared as previously

(10) Image-based and machine learning-guided multiplexed serology test for SARS-CoV-2Cell Reports MethodsAugust 22, 2023Vilja Pietiäinen, Minttu Polso, Ede Migh, Christian Guckelsberger, Maria Harmati, Akos Diosdi, Laura Turunen, Antti Hassinen, Swapnil Potdar, Annika Koponen, et al.Synthetized and cloned to the plasmid by GeneArt/Thermo Fisher Scientific,N/A FuGENE HD,Promega,CAT#E2312 OptiMEM I Reduced Serum Medium,"Gibco® Life Technologies, USA",CAT#31985070 Hoechst 33342,Invitrogen,CAT#H1399 PBS tablets

(11) Two opposing gene expression patterns within ATRX aberrant neuroblastomaPLoS OneAugust 4, 2023Michael R. van Gerven, Linda Schild, Jennemiek van Arkel, Bianca Koopmans, Luuk A. Broeils, Loes A. M. Meijs, Romy van Oosterhout, Max M. van Noesel, Jan Koster, Sander R. van Hooff, et al.(targeting exon 4) and the corresponding homology arm plasmid using Fugene HD transfection reagent (E2312, Promega). Three days after transfection, medium containing 1.5 ug and 1 ug/mL Puromycin (Sigma, P8833)

(12) TMPRSS2 and SARS-CoV-2 SPIKE interaction assay for uHTSJournal of Molecular Cell BiologyAugust 3, 2023Danielle Cicka, Qiankun Niu, Min Qui, Kun Qian, Eric Miller, Dacheng Fan, Xiulei Mo, Andrey A Ivanov, Stefan G Sarafianos, Yuhong Du, et al.HsCD00513790). Transfection HEK-293T cells were transfected using 1 mg/ml polyethyleneimine or FuGENE HD (Promega, E2312) in a ratio of 3 μl to 1 μg DNA and incubated for 48 h. GST pulldown Constructs for GST-TMPRSS2 and

(13) mRNA condensation fluidizes the cytoplasmbioRxivJuly 19, 2023Ying Xie, Tiewei Liu, David Gresham, Liam J Holtlentivirus packaging plasmids psPAX2 and pMD2.G, using fuGENE HD™ transfection reagent (Promega, Cat. no. E2312) following manufacturer’s protocol. 24 hours later, 15 ml antibiotic free DMEM was replaced and the

(14) SIX1 and EWS/FLI1 co-regulate an anti-metastatic gene network in Ewing SarcomaNature CommunicationsJuly 19, 2023Connor J. Hughes, Kaiah M. Fields, Etienne P. Danis, Jessica Y. Hsu, Deepika Neelakantan, Melanie Y. Vincent, Annika L. Gustafson, Michael J. Oliphant, Varsha Sreekanth, Vadym Zaberezhnyy, et al.with pMD2.G and psPAX2 lentiviral packaging plasmids using FuGene HD Transfection Reagent (Promega, E2312) or Lipofectamine 2000 Transfection Reagent (Invitrogen, 11668019). Viral particles were collected at

(15) Affinity and cooperativity modulate ternary complex formation to drive targeted protein degradationNature CommunicationsJuly 13, 2023Ryan P. Wurz, Huan Rui, Ken Dellamaggiore, Sudipa Ghimire-Rijal, Kaylee Choi, Kate Smither, Albert Amegadzie, Ning Chen, Xiaofen Li, Abhisek Banerjee, et al.transiently transfected with the HaloTag-VHL fusion vector using FuGENE HD Transfection Reagent (Promega E2312). The HaloTag-VHL fusion vector contains the coding region for an N-terminal HaloTag fusion to VHL (Promega

(16) 3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM)BIO-PROTOCOLJuly 5, 2023Nuria Ferrandiz, Stephen J. RoyleCorporation, catalog number: P35G-1.5-14-CGRD)Fugene HD transfection reagent (Promega, catalog number: E2312)OptiMEM (Gibco, catalog number: 10149832)GSK923295 (Selleckchem, catalog number: s7090)Thymidine (Sigma,

(17) STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer ProgressionInternational Journal of Molecular SciencesJune 12, 2023Zhike Lin, Ying Liu, Tian Xu, Ting Su, Yingying Yang, Runhua Liang, Songgang Gu, Jie Li, Xuhong Song, Bin Liang, et al.concentration of 1.0 μg/mL using FuGENE HD transfection reagent (Promega Wisconsin, Fitchburg, WI, USA, E2312) according to the manufacturer’s protocol. 4.4. RNA Extraction, Reverse Transcription Polymerase Chain Reaction

(18) Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv : the preprint server for biologyJune 7, 2023Tseng YJ, Malik I, Deng X, Krans A, Jansen-West K, Tank EMH, Gomez NB, Sher R, Petrucelli L, Barmada SJ, et al.effectors were transfected in each well of 96-well and 24-well plate, respectively, with FuGeneHD (Promega, E2312), at a 3:1 ratio of FuGeneHD to DNA. Plasmid reporters were transfected into 70–80% confluent cells

(19) Cross-Platform Synaptic Network Analysis of Human Entorhinal Cortex Identifies TWF2 as a Modulator of Dendritic Spine LengthJ NeurosciMay 17, 2023Courtney K. Walker, Kelsey M. Greathouse, Jennifer J. Tuscher, Eric B. Dammer, Audrey J. Weber, Evan Liu, Kendall A. Curtis, Benjamin D. Boros, Cameron D. Freeman, Jung Vin Seo, et al.constructs were produced by HEK293T cell transfection (catalog #CRL-3216, ATCC) with FugeneHD (catalog #E2312, Promega), OptiMEM (catalog #31985070, Life Technologies), packaging plasmid pxPAX2 (catalog #12260,

(20) RagD auto-activating mutations impair MiT/TFE activity in kidney tubulopathy and cardiomyopathy syndromeNature communicationsMay 15, 2023Sambri I, Ferniani M, Campostrini G, Testa M, Meraviglia V, de Araujo MEG, Dokládal L, Vilardo C, Monfregola J, Zampelli N, et al.co-transfected with either RagD mutants constructs and EGFP empty vector using the Fugene reagent (cat no. E2312 Promega) following the manufacturer’s instructions. 48 h upon transfection, GFP-positive cells were

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